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You will learn how to perform bioinformatic analysis of your DNA sequence to explore the theories about how modern humans evolved and how related you are to your “classmates” and people from around the world. Your DNA will then be sent for sequencing, and results uploaded to the DNALC’s BioServers website. In this workshop you will prepare a sample of your own DNA from cells obtained by cheek swab, use PCR to amplify a section of your own mitochondrial DNA, and confirm the result with agarose gel electrophoresis. These sequence differences, in turn, are the basis for far-ranging investigations on human DNA diversity and evolution. Monday, JPCR and Human DNA Variation: Part 1Ĭomparison of the control region within the human mitochondrial genome reveals that people have distinct patterns of single nucleotide polymorphisms (SNPs). Support for travel for participants outside of commuting distance is available. Faculty who go on to implement in the classroom will also be asked about their experiences and those of their students.
Apply by May 30 th.įaculty are asked to participate in a series of evaluation activities, beginning with workshop evaluation. Travel support and stipends are available for qualified candidates through funding from InnovATEBIO. Recently, Nanopore sequencing was used to deliver the first complete human genome-in time for the 20 th anniversary of the first draft in 2003.ĭuring this workshop we will be actively testing protocols and developing learning materials as part of a concerted effort to make Nanopore sequencing accessible for biology educators. Nanopore’s portability, ability to generate long reads (>1 million bases), and increasing accuracy make it a great choice for DNA and RNA sequencing, DNA modification detection, and metagenomics. The hand-held Oxford Nanopore MinION sequencer can generate up to 50 billion DNA bases in 72 hours for less than $1500.
This five-day workshop will focus on learning materials for educators working with Nanopore sequencing.
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